Two Regions of GerE Required for Promoter Activation in Bacillus subtilis

Two regions of GerE required for promoter activation in Bacillus subtilis.

GerE from Bacillus subtilis is the smallest member of the LuxR-FixJ family of transcription activators. Its 74-amino-acid sequence is similar over its entire length to the DNA binding domain of this protein family, including a putative helix-turn-helix (HTH) motif. In this report, we sought to define regions of GerE involved in promoter activation. We examined the effects of single alanine subs...

A Region of s Involved in Promoter Activation by GerE in Bacillus subtilis

During endospore formation in Bacillus subtilis, the DNA binding protein GerE stimulates transcription from several promoters that are used by RNA polymerase containing s. GerE binds to a site on one of these promoters, cotX, that overlaps its 235 region. We tested the model that GerE interacts with s at the cotX promoter by seeking amino acid substitutions in s that interfered with GerE-depend...

Promoter Activation in Bacillus subtilis

Activation in Bacillus subtilis

During sporulation, Bacillus subtilis undergoes an asymmetric division that results in two cells with different fates, the larger mother cell and the smaller forespore. The protein phosphatase SpoIIE, which is required for activation of the forespore-specific transcription factor , is also required for optimal efficiency and timing of asymmetric division. We performed a genetic screen for spoII...

MOLECULAR CLONING AND EVALUATION OF WILD PROMOTER IN EXPRESSION OF BACILLUS SPHAERICUS PHENYLALANINE DEHYDROGENASE GENE IN BACILLUS SUBTILIS CELLS

To evaluate the role of wild promoter of L-phenylalanine dehydrogenase (PheDH) gene, referred to as pdh, from Bacillus sphaericus in expression, cloning of pdh gene in Bacillus subtilis was performed. The whole pdh gene was cloned in pHY300PLK shuttle vector and amplified, construct (pHYDH) then transformed in B. subtilis ISW1214 and E. coli JM109. The pdh endogenous promoter presented no effec...